Transient Complexes between Dark Rhodopsin and Transducin: Circumstantial Evidence or Physiological Necessity?
نویسندگان
چکیده
منابع مشابه
Complexes between photoactivated rhodopsin and transducin: progress and questions.
Activation of GPCRs (G-protein-coupled receptors) leads to conformational changes that ultimately initiate signal transduction. Activated GPCRs transiently combine with and activate heterotrimeric G-proteins resulting in GTP replacement of GDP on the G-protein alpha subunit. Both the detailed structural changes essential for productive GDP/GTP exchange on the G-protein alpha subunit and the str...
متن کاملThe rhodopsin-transducin complex houses two distinct rhodopsin molecules.
Upon illumination the visual receptor rhodopsin (Rho) transitions to the activated form Rho(∗), which binds the heterotrimeric G protein, transducin (Gt) causing GDP to GTP exchange and Gt dissociation. Using succinylated concanavalin A (sConA) as a probe, we visualized native Rho dimers solubilized in 1mM n-dodecyl-β-d-maltoside (DDM) and Rho monomers in 5mM DDM. By nucleotide depletion and af...
متن کاملProbing rhodopsin–transducin interaction using Drosophila Rh1–bovine rhodopsin chimeras
Invertebrate and vertebrate rhodopsins share a low degree of homology and are coupled to G-proteins from different families. Here we explore the utility of fly-expressed chimeras between Drosophila rhodopsin Rh1 and bovine rhodopsin (Rho) to probe the interactions between the invertebrate and vertebrate visual pigments and their cognate G-proteins. Chimeric Rh1 pigments carrying individual subs...
متن کاملNMR analysis of rhodopsin–transducin interactions
Heterotrimeric G-protein activation by an agonist-stimulated G-protein coupled receptor (R*) requires the propagation of structural signals from the receptor interacting surfaces to the guanine nucleotide-binding pocket. Employing high-resolution NMR methods, we are probing heterotrimer-associated and rhodopsin-stimulated changes in an isotope-labeled G-protein alpha-subunit (G(alpha)). A key a...
متن کامل[Vertical or circumstantial transmission?].
Si bien es una gran limitante el no haber realizado los exámenes complementarios mencionados (aislamiento viral, los hallazgos histopatológicos en la placenta), hubiese sido de gran valor el aislamiento del virus en aspirado traqueo-bronquial, ya que este es más específico (2). El hallazgo de un PCR positivo en un hisopado nasal dentro del contexto de una pandemia no necesariamente indica que e...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2015
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2014.12.031